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We routinely test our siRNAs to see if they induce any of 3 or 4 interferon-inducible genes, and compare to our standard -ve control. I am almost certain that we tested that particular GFP control and decided not to use it, but I can't remember if interferon induction was the reason (my lab notes for those experiments are elsewhere). On the other hand I know we tested a different GFP -ve control that induced sky-high levels of interferon.

Off-target effects due to interferon induction is a huge concern and something that needs to be specifically tested in every siRNA experiment.

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