« Dysfunctional CD8 T cells in HIV Infection: Helpless & Unhelpful | Main | Case Report Suggests Antiretroviral Prophylaxis May Have Attenuated HIV Infection »

Comments

iayork

We routinely test our siRNAs to see if they induce any of 3 or 4 interferon-inducible genes, and compare to our standard -ve control. I am almost certain that we tested that particular GFP control and decided not to use it, but I can't remember if interferon induction was the reason (my lab notes for those experiments are elsewhere). On the other hand I know we tested a different GFP -ve control that induced sky-high levels of interferon.

Off-target effects due to interferon induction is a huge concern and something that needs to be specifically tested in every siRNA experiment.

Verify your Comment

Previewing your Comment

This is only a preview. Your comment has not yet been posted.

Working...
Your comment could not be posted. Error type:
Your comment has been saved. Comments are moderated and will not appear until approved by the author. Post another comment

The letters and numbers you entered did not match the image. Please try again.

As a final step before posting your comment, enter the letters and numbers you see in the image below. This prevents automated programs from posting comments.

Having trouble reading this image? View an alternate.

Working...

Post a comment

Comments are moderated, and will not appear until the author has approved them.

Your Information

(Name and email address are required. Email address will not be displayed with the comment.)

Other Accounts

Search this blog


January 2023

Sun Mon Tue Wed Thu Fri Sat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31        
Blog powered by Typepad
Member since 09/2005