The July issue of the journal Cytometry Part A contains an excellent article by Pratip Chattopadhyay and Mario Roederer reviewing the use of flow cytometry to study T cell subsets in HIV infection. The entire journal issue is free access.
Cytometry Part A
Special Issue: CYTOMETRY - 30th Anniversary 1980 – 2010
Volume 77A, Issue 7, pages 614–622, July 2010
Pratip K. Chattopadhyay, Mario Roederer
Flow cytometry is a key technology in the study of HIV disease. In this article, we review various cellular markers that can be measured in the setting of pathogenesis or vaccination studies, including markers of activation, differentiation, senescence, immune suppression, and function. In addition, we discuss important considerations for making these measurements. Finally, we examine how flow cytometry studies have taught researchers about the disease process, and the potential for flow cytometry technology to guide treatment decisions and evaluate vaccine candidates in the future.